Effect of a single resistance exercise session on inflammatory markers and oxidative stress in women living with HIV / Efeito de uma única sessão de exercício resistido sobre marcadores inflamatórios e estresse oxidativo em mulheres vivendo com hiv

ABSTRACT People living with HIV (PLH), who use antiretroviral therapy (ART), are more susceptible to changes in the inflammatory profile and oxidative stress, and women have greater access to ART. Although physical exercise is a complementary strategy to treatment due to its antioxidant and anti-inflammatory effects, it is not clear whether acute responses to exercise can be harmful to PLH. The aim of the study was to investigate the acute effect of resistance exercise (RE) on inflammatory and oxidative stress markers in PLH. Ten women, using ART, performed RE session consisting of seven exercises for the whole body. For biochemical evaluation, blood samples were collected before (pre), 1 hour (1h) and 2 hours (2h) after the RE session. One-way ANOVA followed by Bonferroni's post hoc test was used to compare results between time points. There was an increase only in markers, GSSG of 160% (pre: 0.40 ± 0.11; 1h: 1.18 ± 0.36; 2h: 1.04 ± 0.25 mmol/g), TNF-α of 98 % (pre: 4.60 ± 0.55; 1h: 6.95 ± 0.77; 2h: 9.10 ± 1.03 pg/ml) and 52% IL-6 (pre: 2.47 ± 0 .67; 1h: 3.63 ± 1.26; 2h: 5.38 ± 2.15 pg/ml). The other variables remained unchanged (P > 0.05). It is concluded that a RE session increased the levels of inflammatory markers and oxidative stress in PLH in a non-exacerbated way.

RESUMO Pessoas vivendo com HIV (PVH), que utilizam a terapia antirretroviral (TARV), são mais suscetíveis a alterações no perfil inflamatório e estresse oxidativo, sendo que as mulheres possuem maior acesso à TARV. Embora o exercício físico seja uma estratégia complementar ao tratamento devido aos seus efeitos antioxidantes e anti-inflamatórios, não está claro se as respostas agudas ao exercício podem ser prejudiciais às PVH. O objetivo do estudo foi investigar o efeito agudo de exercícios com pesos (EP) sobre marcadores inflamatórios e de estresse oxidativo em PVH. Dez mulheres, em uso da TARV, realizaram uma sessão de EP constituída por sete exercícios para o corpo todo. Para avaliação bioquímica, amostras de sangue foram coletadas antes (pré), 1 hora (1h) e 2 horas (2h) após a sessão de EP. A ANOVA one-way seguida do teste post hoc de Bonferroni foi utilizada para comparação dos resultados entre os momentos. Houve aumento apenas nos marcadores, GSSG de 160% (pré: 0,40 ± 0,11; 1h: 1,18 ± 0,36; 2h:1,04 ± 0,25 mmol/g), TNF-α de 98% (pré: 4,60 ± 0,55; 1h: 6,95 ± 0,77; 2h: 9,10 ± 1,03 pg/ml) e IL-6 de 52% (pré: 2,47 ± 0,67; 1h: 3,63 ± 1,26; 2h: 5,38 ± 2,15 pg/ml). As demais variáveis permaneceram sem alterações (P > 0,05). Conclui-se que uma sessão de EP aumentou os níveis de marcadores inflamatórios e estresse oxidativo em PVH de forma não exacerbada.

Impacto do treinamento resistido na distribuição de gordura de pessoas com HIV: ensaio clínico randomizado / Impact of resistance training on fat distribution in people living with HIV: randomized clinical trial

Verificar o impacto de oito semanas de treinamento resistido (TR) na distribuição de gordura corporal de pessoas vivendo com o vírus da imunodeficiência humana (HIV) (PVH). Participaram 17 PVHs aleatorizadas em dois grupos: controle (GC, n=6) e treino (GT, n=11). O protocolo de TR foi composto por dez exercícios. A avaliação das dobras cutâneas (DC) tricipital, subescapular, abdominal, coxa e perna dos indivíduos foi realizada antes e depois do período de treinamento. As análises indicaram diferenças significantes (p<0,05) entre os grupos após o período de intervenção, com diminuição de gordura subcutânea no GT e aumento no GC tanto para as DCs da região do tronco (-3,77%, 12,97%) quanto dos membros (-10,28%, 10,08%). No somatório das DCs, o GT teve uma redução média de 6,43%, enquanto o GC aumentou 11,12% após as oito semanas, com diferenças significantes entre os grupos (p<0,05). Oito semanas de TR diminuíram a gordura corporal subcutânea de PVHs.

To verify the impact of eight weeks of resistance training (RT) on body fat distribution of people living with human immunodeficiency virus (HIV) (PLH). Seventeen randomized PLH participated in two groups: control (CG, n=6) and training (TG, n=11). The protocol of RT consisted of ten exercises. The subjects' triceps, subscapular, abdominal, thigh, and leg skinfolds were assessed before and after the training period. The analysis indicated significant differences (p<0.05) between the groups after the period of intervention, with decreased subcutaneous fat in the TG and increased subcutaneous fat in the CG in the areas of the torso (-3.77%, 12.97%) and limbs (-10.28%, 10.08%). By calculating the sum of skinfolds, the TG had an average reduction of 6.43%, while the CG increased by 11.12% after the eight weeks, with significant differences between the groups (p<0.05). Eight weeks of RT decreased subcutaneous body fat in PLH.

Effect of Creatine Supplementation on Functional Capacity and Muscle Oxygen Saturation in Patients with Symptomatic Peripheral Arterial Disease: A Pilot Study of a Randomized, Double-Blind Placebo-Controlled Clinical Trial.

The aim of the study was to verify the effects of creatine (Cr) supplementation on functional capacity (walking capacity; primary outcome) and calf muscle oxygen saturation (StO2) (secondary outcome) in symptomatic peripheral arterial disease (PAD) patients. Twenty-nine patients, of both sexes, were randomized (1:1) in a double-blind manner for administration of placebo (PLA, n = 15) or creatine monohydrate (Cr, n = 14). The supplementation protocol consisted of 20 g/day for 1 week divided into four equal doses (loading phase), followed by single daily doses of 5 g in the subsequent 7 weeks (maintenance phase). Functional capacity (total walking distance) was assessed by the 6 min walk test, and calf muscle StO2 was assessed through near infrared spectroscopy. The measurements were collected before and after loading and after the maintenance phase. The level of significance was p < 0.05. No significant differences were found for function capacity (total walking distance (PLA: pre 389 ± 123 m vs. post loading 413 ± 131 m vs. post maintenance 382 ± 99 m; Cr: pre 373 ± 149 m vs. post loading 390 ± 115 m vs. post maintenance 369 ± 115 m, p = 0.170) and the calf muscle StO2 parameters (p > 0.05). Short- and long-term Cr supplementation does not influence functional capacity and calf muscle StO2 parameters in patients with symptomatic PAD.

Influence of the order of aerobic and resistance exercise on hemodynamic responses and arterial stiffness in young normotensive individuals.

AIMS: To investigate the effects of a combination of aerobic and resistance exercises and the inverse sequence on the hemodynamic parameters and indicators of arterial stiffness in healthy young adult subjects. METHODS: Fifteen subjects were randomized in a crossover procedure according to two experimental conditions: combined aerobic exercise (30 min of treadmill running, 75-80% - peak VO2) followed by resistance exercise (5 exercises, 3 sets - 10 RM) (AR) or vice versa (RA). Data of the hemodynamic parameters and arterial stiffness were obtained at baseline and after exercise (post-10, post-20, and post-30 min). Two-way ANOVA for repeated measurements was performed with the Newman-Keuls post-hoc. The significance level adopted was p < 0.05. RESULTS: The results of the two-way ANOVA for repeated measures were not statistically significant for brachial and central systolic and diastolic blood pressure, respectively, or arterial stiffness indicators: reflected wave indicators and pulse wave velocity (P > 0.05). Statistically significant interactions were observed before and after the exercise sessions for heart rate and rate pressure product (P = < 0.001). CONCLUSION: The performance order of aerobic exercise followed by resistance exercise (AR) and the reverse order (RA) present similar changes in blood pressure (BP) and arterial stiffness. However, resistance exercise before aerobic exercise promotes increases in heart rate and rate product pressure.

O período do dia não influencia o desempenho físico de homens praticantes de treinamento com pesos / Day period fails to affect the physical performance of males who practice weight lifting

O objetivo deste estudo é avaliar o desempenho de praticantes de treinamento com pesos em diferentes períodos do dia. A amostra foi composta por 10 homens treinados (24,4 ± 2,7anos, 80,6 ± 12,7 kg, 180,0 ± 10,8 cm), submetidos ao teste de uma repetição máxima (1RM) para determinação de carga máxima, no exercício supino no banco horizontal. Em seguida, três encontros foram destinados para realização do teste de resistência de força a 80% de 1RM, realizados em diferentes períodos do dia, de maneira aleatória. A análise de variância (ANOVA one-way) foi utilizada para verificar as médias dos grupos nos períodos distintos. Não foram encontradas diferenças no desempenho, independente do horário do dia, manhã (25 ± 4 repetições), tarde (26 ± 7 repetições) e noite (26 ± 7 repetições) em relação ao desempenho obtido (P > 0,05). Assim, sugere-se que o período do dia não influencia o desempenho da força e fadiga de indivíduos jovens treinados.

To evaluate the performance of weight lifterS at different periods of the day. Method: sample comprised 10 trained males (24.4± 2.7 years, 80.6±12.7kg, 180.0±10.8cm) who underwent maximum repetition test (MRT) to determine maximum load, by supine exercise, on a horizontal bench. Three sessions were employed to test force resistance at 80% MRT, at random, at different periods of the day. One way ANOVA was employed to verify means of groups within distinct periods. There was no difference in performance with regard to day period: morning (25 ± 4 repetitions), afternoon (26 ± 7 repetitions) and evening (26 ± 7 repetitions) with regard to performance (P > 0.05). It may be suggested that the period of the day does not affect force performance and fatigue of trained young people.

Does Creatine Supplementation Affect Renal Function in Patients with Peripheral Artery Disease? A Randomized, Double Blind, Placebo-controlled, Clinical Trial.

BACKGROUND: Case studies and reviews have shown that creatine supplementation can affect kidney function. The objective of this study is to verify the effects of 8 weeks of creatine supplementation on renal function (creatinine clearance: primary outcome) in patients with symptomatic peripheral arterial disease. METHODS: Twenty-nine patients, of both genders, were randomized (1:1) in a double-blind manner for administration of Placebo (PLA; n = 15) or creatine monohydrate (Cr; n = 14). The supplementation protocol consisted of 20 g/day for 1 week divided into 4 equal doses (loading phase), followed by single daily doses of 5 g in the subsequent 7 weeks (maintenance phase). Before and after the supplementation period, markers of renal function, serum creatinine, creatinine excretion rate, and creatinine clearance were evaluated. The Generalized Estimation Equation Model was used for comparison between groups. The level of significance was P < 0.05. RESULTS: No significant differences were found between groups before and after the intervention for serum creatinine (Cr: pre 1.00 ± 0.15 mL/dL vs. post 1.07 ± 0.16 mL/dL; PLA: pre 1.30 ± 0.53 mL/dL vs. post 1.36 ± 0.47 mL/dL, P = 0.590), creatinine excretion rate (Cr: pre 81.73 ± 43.80 mg/dL vs. post 102.92 ± 59.57 mg/dL; PLA: pre 74.37 ± 38.90 mg/dL vs. post 86.22 ± 39.94 mg/dL, P = 0.560), or creatinine clearance (Cr; pre 108 ± 59 mL/min/1.73 m2 vs. post 117 ± 52 mL/min/1.73 m2; PLA: pre 88 ± 49 mL/min/1.73 m2 vs. post 82 ± 47 mL/min/1.73 m2, P = 0.366). CONCLUSIONS: Eight weeks of creatine supplementation is safe and does not compromise the renal function of patients with peripheral arterial disease.